Soil Microbiome Analysis & Reporting Tool
Upload your R1 & R2 sequencing files and get results in minutes — no coding required.
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Analysis Pipeline: AMPtk
OTU Clustering
🍄 AMPtk — Fungal ITS2 OTU Clustering
· Designed for fungal ITS2 amplicon sequencing (Illumina MiSeq paired-end)
· Quality filtering → OTU clustering at 97% similarity → Taxonomy assignment
· Uses VSEARCH for clustering and SINTAX for taxonomy (ITS2 database)
· Fast, stable, and well-validated pipeline
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Upload Sequencing Data
Illumina MiSeq paired-end reads
Upload R1 File
*_R1_001.fastq or .fastq.gz
Upload R2 File
*_R2_001.fastq or .fastq.gz
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Analysis Options
Optional — default values recommended
I have a Mock Barcode (spike-in control)
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Run Analysis